Invitrogen™ Human ST3GAL1 ELISA Kit

Description

ELISA

Human ST3GAL1 quantitates human ST3GAL1 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human ST3GAL1.

ST3GAL1 is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. It is normally found in the Golgi but can be proteolytically processed to a soluble form. Correct glycosylation of ST3GAL1 protein may be critical to its sialyltransferase activity. This protein, which is a member of glycosyltransferase family 29, can use the same acceptor substrates as does sialyltransferase 4B.The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein is normally found in the Golgi but can be proteolytically processed to a soluble form. Correct glycosylation of the encoded protein may be critical to its sialyltransferase activity. This protein, which is a member of glycosyltransferase family 29, can use the same acceptor substrates as does sialyltransferase 4B. Two transcript variants encoding the same protein have been found for this gene. Other transcript variants may exist, but have not been fully characterized yet.

Specifications

• Accession Number: 5330418N22Rik; AI467004; Alpha 2,3-ST 1; beta-galactoside alpha-2,3-sialyltransferase 1; CMP-N-acetylneuraminate: [beta-galactosidase alpha-2,3 ] sialytransferase; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1; Gal beta1,3 GalNAc alpha2,3 sialyltransferase; Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase; Gal-NAc6S; MGC9183; Sialyltransferase 4 A; sialyltransferase 4 A (beta-galactosidase alpha-2,3-sialytransferase); sialyltransferase 4 A (beta-galactoside alpha-2,3-sialyltransferase); sialyltransferase 4 A (beta-galactoside alpha-2,3-sialytransferase); SIAT4; SIAT4A; SIAT4-A; SIATFL; ST3 beta-galactoside alpha-2,3-sialyltransferase 1; ST3Gal I; St3gal1; St3gal-1; ST3GalA; ST3GalA0.1; ST3GalI; ST3GAL-I sialyltransferase; ST3GalIA; ST3GalIA,1; ST3O
• Assay Range: 1.8 to 400 ng/mL
• Assay Sensitivity: 1.8 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 6482
• Gene Symbol: ST3GAL1
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: ST3GAL1
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins

Safety and Handling

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