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Invitrogen™ TNFR1 (Soluble) Human Instant ELISA™ Kit

Instant ELISA Kit

Manufacturer:  Invitrogen™ BMS203INST


Catalog No. 50-179-35

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Includes: Aluminium pouch(es) with a Microwell Plate coated with Monoclonal Antibody (murine) to human sTNF-R (60 kDa) and HRP-Conjugate (anti-sTNF-R (60 kDa) monoclonal (murine) antibody), lyophilized
Aluminium pouch(es) with a human sTNF-R (60kDa) Standard curve (coloured)
Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Assay Buffer Concentrate 20x (Use when an external predilution of the samples is needed)
Stop Solution (1M Phosphoric acid)
Adhesive Plate Covers



The Human Soluble Tumor Necrosis Factor Receptor 1(Hu sTNRF1) ELISA quantitates Hu sTNFR1 in human serum, plasma, cell culture supernatants, or other body fluids. The assay will exclusively recognize both natural and recombinant Hu sTNFR. Principle of the method The Human sTNFR1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Tumor necrosis factor receptor 1 (TNFR1) belongs to the tumor necrosis factor superfamily, and is one of the major TNF-alpha receptors. This receptor plays an important role in mediating apoptosis. TNFR1 also plays an important role in cytokine mediated signaling, positive regulation of the NF-Kb pathway, positive regulation of angiogenesis, and negative regulation of gene expression. The extracellular domain of the receptor is also released into the circulatory system as soluble TNFR1 (sTNFR1). In humans, the TNFR1 gene is located on chromosome 12.


Colorimetric Microplate Reader
Pre-coated 96 well Microplate, Detection Antibody, Anti-IgG-HRP, Sample Diluent, lyophilized, Standard curve strips, Wash Buffer Concentrate, Substrate Solution, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers
Plasma, 10 μL; Serum, 10 μL; Supernatant, 10 μL
20 min.
128 Tests
Plasma, Serum, Supernatant
0.078-5.0 ng/mL
2 hr. 10 min.
Tumor necrosis factor receptor 1, TNF-R1, CD120a
53 pg/mL
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