Speed: Sample to actionable data by allowing multiple assays to be run in one assay thereby reducing the hands-on time numbers of overall tests and conserving precious samples.Results: 11 endpoints from a single-well assay measuring T cell exhaustion phenotypes and function as well as cytokine secretion in response to stimulation cell viability and an optional proliferation dye allows screening for molecules that reverse T cell exhaustion.Biological insight: Generate functional data and analyze cell health from the same well by multiplexing cell and cytokine detection in one assay.Simplicity: Single platform assay data acquisition and analysis eliminate the time of acquiring and correlating data from multiple sources.