Speed: Sample to actionable data by allowing multiple assays to be run in one assay thereby reducing the hands on time numbers of overall tests and conserving precious sample.Results: 8 endpoints from a single well assay measuring markers of T cell immunophenotypes and function cell health cell count secreted pro-inflammatory cytokine IFNγ and pro-apoptotic serine protease Granzyme B.Biological insight: Generate functional data and analyze cell health from same cell by multiplexing cell phenotypes and function in a single well.Simplicity: Single platform assay and data acquisition eliminates the time of acquiring and correlating data from multiple sources.