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Gibco™ Human TRAIL (TNFSF10) Recombinant Protein

Catalog No. PHC1634
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Quantity:
50 μg

Recombinant Protein

Carrier-Free Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile, distilled water to a concentration of 0.5-1.0 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C. Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage, preferably desiccated. Working aliquots stored with a carrier protein are stable for at least 12 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.

TRAIL is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. TRAIL preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of TRAIL protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Receptors for TRAIL include two death domain containing receptors, death receptor 4 (DR4) and DR5, as well as two decoy receptors, DcR1 and DcR2, lacking the intracellular signaling death domain. TRAIL is a type II membrane protein and expressed in a variety of human tissues. Like TNF and Fas ligand, TRAIL induces apoptosis and NF-kB activation in many tissues and cells.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P50591
For Use With (Application) Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
Formulation 10 mM sodium phosphate with 110 mM NaCl and no preservative; pH 7.2
Gene ID (Entrez) 8743
Molecular Weight (g/mol) 19.6 kDa
Name Human TRAIL (TNFSF10)
Purification Method Sequential Chromatography
Quantity 50 μg
Endotoxin Concentration <0.1 ng/ μg
Gene Alias A330042I21Rik; AI448571; Apo-2 ligand; APO2L; Apo-2 L; CD253; CD253 antigen; chemokine tumor necrosis factor ligand superfamily member 10; Ly81; Protein TRAIL; TL2; TN; TNF superfamily member 10; TNF-related apoptosis inducing ligand; TNF-related apoptosis inducing ligand TRAIL; TNF-related apoptosis-inducing ligand; Tnfsf10; TNLG6A; TRAIL; TRAIL protein; TRAIL/APO2L; tumor necrosis factor (ligand) family, member 10; tumor necrosis factor (ligand) superfamily member 10; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor apoptosis-inducing ligand splice variant delta; tumor necrosis factor ligand 6 A; tumor necrosis factor ligand superfamily member 10; tumor necrosis factor superfamily member 10
Common Name TRAIL (CD253)
Biological Activity ED50 = 1 - 3 ng/mL; determined by the dose-dependent apoptosis of U34MG cells.
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Sequence Human TRAIL recombinant protein contains 168 amino acids
Content And Storage -20°C
Classification Carrier-Free
Endotoxin Level <0.1 ng/μg
Shipping Condition Approved for shipment on Wet or Dry Ice
Expression System E. coli
Protein Family Cytokines & Receptors
Protein Form Recombinant, Ligand
Form Lyophilized
Protein Subtype Other Proteins
Research Category Apoptosis, Oncology, Signal Transduction
Product Line Gibco™
Purity or Quality Grade ≥98% by SDS-PAGE and HPLC
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WARNING: Cancer - www.P65Warnings.ca.gov
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