Gibco™ Human TRANCE (RANKL) (soluble) Recombinant Protein, PeproTech®

Description

Recombinant Protein

PeproTech's CHO cell-derived Recombinant Human sRANK Ligand is a 22.1 kDa polypeptide comprised of the TNF-homologous region of RANKL and a N-terminal His-tag. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.

Specifications

• Accession Number: O14788
• For Use With (Application): Functional Assay
• Formulation: protein with no preservative
• Gene ID (Entrez): 8600
• Molecular Weight (g/mol): 22.1 kDa
• Name: Human TRANCE (RANKL) (soluble)
• Quantity: 100 μg
• Source: CHO cells
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: CD254; H-RANK-L; hRANKL2; Ly109l; M-Rank-L; ODF; OPG; OPG ligand; OPGL; OPTB2; Osteoclast differentiation factor; osteoprotegerin ligand; Rankl; rank-l; receptor activator of NF-kappaB ligand; receptor activator of nuclear factor kappa B ligand; receptor activator of nuclear factor kappa-B ligand; RP11-86N24.2; sOdf; srankl; TNF-related activation-induced cytokine; Tnfsf11; TNLG6B; Trance; tumor necrosis factor (ligand) superfamily member 11; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand 6 B; tumor necrosis factor ligand superfamily member 11; Tumor necrosis factor ligand superfamily member 11, membrane form; Tumor necrosis factor ligand superfamily member 11, soluble form; tumor necrosis factor superfamily member 11; tumor necrosis factor-related activation-induced cytokine
• Common Name: RANKL (RANK Ligand)
• Gene Symbol: TNFSF11
• Biological Activity: Determined by its dose-dependent ability to induce reporter gene in HT-29 NF-kappaB Luc reporter cells.
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: HHHHHHHHPS PGGSGGSQHI RAEKAMVDGS WLDLAKRSKL EAQPFAHLTI NATDIPSGSH KVSLSSWYHD RGWGKISNMT FSNGKLIVNQ DGFYYLYANI CFRHHETSGD LATEYLQLMV YVTKTSIKIP SSHTLMKGGS TKYWSGNSEF HFYSINVGGF FKLRSGEEIS IEVSNPSLLD PDQDATYFGA FKVRDID
• Content And Storage: -20°C
• Expression System: CHO cells
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.