Invitrogen™ Human Trypsin Pan/PRSS1-2-3 ELISA Kit

Description

ELISA

Human Trypsin Pan/PRSS1-2-3 quantitates human Trypsin Pan/PRSS1-2-3 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Trypsin Pan/PRSS1-2-3.

Trypsin is a 24 kDa enzyme of the serine proteinase family. It is produced in the pancreas as an inactive precursor, trypsinogen, but the active enzyme is located in the gastrointestinal tract where it degrades proteins to large peptides. Trypsin prefers lysine and arginine residues. One of the substrates for trypsin is chymotrypsinogen which is cleaved to produce active chymotrypsin. High levels of immunoreactive trypsin in the bloodstream can indicate pancreatic malfunction and this indicator is used to screene for and diagnose Cystic Fibrosis.

Specifications

• Accession Number: Alpha-trypsin chain 1; Alpha-trypsin chain 2; Anionic trypsinogen; beta-trypsin; Brain trypsinogen; cationic trypsinogen; digestive zymogen; mesotrypsin; Mesotrypsinogen; MTG; nonfunctional trypsin 1; pancreatic trypsinogen III; protease serine 2 preproprotein; protease, serine 1; protease, serine 2; protease, serine 3; protease, serine, 2 (trypsin 2); protease, serine, 4 (trypsin 4, brain); PRSS1; PRSS2; PRSS3; PRSS4; Serine protease 1; Serine protease 2; Serine protease 3; Serine protease 4; T9; TCR V beta 4.1; TRP1; TRY1; TRY2; TRY3; TRY4; TRY8; TRYP1; TRYP2; Trypsin I; Trypsin II; trypsin III; Trypsin IV; trypsin-1; Trypsin-2; Trypsin-3; trypsinogen 1; trypsinogen 2; trypsinogen 4; trypsinogen 5; trypsinogen A; trypsinogen IV
• Assay Range: 30.72 to 7,500 pg/mL
• Assay Sensitivity: 30 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 5644, 5645, 5646
• Gene Symbol: PRSS1, PRSS2, PRSS3
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: Trypsin Pan
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov