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Invitrogen™ TWEAK Human Instant ELISA™ Kit Non-distribution product as customer accommodation.

Instant ELISA Kit

Manufacturer:  Invitrogen™ BMS2006INST

Catalog No. 50-112-8811


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Includes: Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to human TWEAK, Biotin-Conjugate (anti-human TWEAK polyclonal antibody), Streptavidin-HRP and Sample Diluent, lyophilized
Aluminium pouch(es) with a human TWEAK Standard curve (coloured)
Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
Sample Diluent (Use when an external predilution of the samples is needed)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films

Description

Description

The Human Tumor necrosis factor-like WEAK inducer of apoptosis (Hu TWEAK) ELISA quantitates Hu TWEAK in human serum, plasma, cell culture supernatants, urine, amniotic fluid, bile, or other body fluids. The assay will exclusively recognize both natural and recombinant Hu TWEAK. Principle of the method The Human TWEAK solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

TNF-related weak inducer of apoptosis (TWEAK) is a member of the tumor necrosis factor superfamily (TNFSF) of structurally related cytokines. Like most other members of this family, TWEAK is a cell surface-associated type II transmembrane protein although a smaller, biologically active form can also be generated by cleavage near the cell membrane. TWEAK has multiple biological activities, including stimulation of cell growth and angiogenesis, induction of inflammatory cytokines, in addition to stimulation of apoptosis. The TWEAK signal transduction pathway has not been well established but it appears to signal via TweakR/Fn14 in a manner similar to that described for other TNFSF members that bind receptors lacking death domains.
Specifications

Specifications

O43508
Biotin
Colorimetric Microplate Reader
Pre-coated 96 well Microplate, Biotinylated Detection Antibody, SAV-HRP, Sample Diluent, lyophilized, Standard curve strips, Wash Buffer Concentrate, Substrate Solution, Sample Diluent, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers
RUO
Plasma, Serum, Supernatants
-20°C
3 hr 10 min
Human
TNFSF12
7.9%
ELISA Kit
15.6-1,000 pg/ml
8742
ELISA
ELISA
9.7 pg/ml
Plasma (50 μl), Serum (50 μl), Supernatants (50 μl)
20 min
128 tests
Plasma,50 μL|Serum,50 μL|Supernatant,50 μL
9.2%
HRP
Documents
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