R&D Systems™ Recombinant Human UbcH5a/UBE2D1 - Ubiquitin Charged Protein

Catalog No.	Quantity
E2800100	100 μg

Catalog No. E2800100 Supplier R&D Systems™ Supplier No. E2800100

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Pre-charged UBE2D1 may be added directly to in vitro reactions containing E3 Ubiquitin ligases and ligase substrates (if applicable)—no ATP, E1 enzyme, or extra Ubiquitin are required. Reaction conditions will need to be optimized for each specific application. Note: Reducing agents including dithiothreitol (DTT) or mercaptoethanol ( beta ME) may cause unintended thiolytic release of Ubiquitin from the complex—care must be taken if these compounds are present in buffers. We suggest using a thioester friendly reductant such as TCEP if possible.

Specifications

• Accession Number: P51668, P0CG47
• For Use With (Application): Bioactivity
• Formulation: Approximately 0.43 mg/mL (25 μM) with respect to UBE2D1. Formulated in 50 mM HEPES pH 7.5, 50 mM NaCl, 1 mM TCEP
• Gene ID (Entrez): 7321
• Molecular Weight (g/mol): M.W. (theoretical): 25 kDa (17 kDa UBE2D1, 8.6 kDa Ubiquitin)
• Quantity: 100 μg
• Source: E. coli-derived human UbcH5a/UBE2D1 protein
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Gene Alias: E2(17)KB1, Stimulator of Fe transportUBC4/5 homolog, UBC4/5, UBC5A, UbcH5, UbcH5A, UBCH5AEC 6.3.2.19, UBCH5SFT, UBE2D1, Ubiquitin carrier protein D1, ubiquitin-conjugating enzyme E2 D1, Ubiquitin-conjugating enzyme E2(17)KB 1, Ubiquitin-conjugating enzyme E2-17 kDa 1, ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast), Ubiquitin-protein ligase D1
• Common Name: UbcH5a/UBE2D1
• Gene Symbol: UBE2D1
• Biological Activity: Pre-charged UBE2D1 may be added directly to in vitro reactions containing E3 Ubiquitin ligases and ligase substrates (if applicable)—no ATP, E1 enzyme, or extra Ubiquitin are required. Reaction conditions will need to be optimized for each specific application. Note: Reducing agents including dithiothreitol (DTT) or mercaptoethanol ( beta ME) may cause unintended thiolytic release of Ubiquitin from the complex—care must be taken if these compounds are present in buffers. We suggest using a thioester friendly reductant such as TCEP if possible.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: E. coli-derived Accession # P51668, P0CG47
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain.
• Protein: UbcH5a/UBE2D1