Abnova™ Human VEGFA (165 a.a.) Full-length Recombinant Protein with His tag expressed in Barley grain

Catalog No.	Quantity
89-966-998	1 mg

Catalog No. 89-966-998 Supplier Abnova™ Supplier No. P5816

Description

Used for Func, SDS-PAGE, WB-Re

This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq]

Specifications

• Accession Number: NP_001165097
• Concentration: 100μg/mL
• For Use With (Application): Functional Assay, SDS-PAGE, Western Blot
• Formulation: Lyophilized from PBS, pH 7.2
• Gene ID (Entrez): 7422
• Molecular Weight (g/mol): 30kDa
• Name: Human VEGFA (165 a.a.) Full-length Recombinant Protein with His tag expressed in Barley grain
• Purification Method: Chromatography
• Quality Control Testing: SDS-PAGE Stained with Coomassie Blue
• Quantity: 1 mg
• Storage Requirements: Store at −20°C on dry atmosphere.After reconstitution with sterile water to a concentration of no less than 100 ug/mL, store at −20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Aliquot to avoid repeated freezing and thawing.
• Regulatory Status: RUO
• Endotoxin Concentration: <0.005ng/μg protein (0.05EU/μg) as measured by turbidimetric kinetic assay
• Gene Alias: MGC70609, VEGF, VEGF-A, VPF
• Common Name: VEGFA
• Gene Symbol: VEGFA
• Biological Activity: The ED50 is determined by Cell Proliferation Assay for the dose-dependent effects of serial dilutions of recombinant human VEGFA on HUVEC cells. The ED50 value was determined to be 0.58ng/mL.
• Species: Plants
• Recombinant: Recombinant
• Protein Tag: His Tag
• Expression System: Barley grain (Hordeum vulgare) expression system
• Form: Lyophilized
• Purity or Quality Grade: >95% by SDS-PAGE