Invitrogen™ Human VEGFR3 (Flt-4) His-tag Recombinant Protein

Catalog No.	Quantity
PIRP88181	100 μg
PIRP88182	1 mg

Catalog No. PIRP88181 Supplier Invitrogen™ Supplier No. RP88181

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. Predicts a molecular mass of 86 kDa. As a result of glycosylation, rhVEGFR3 migrates as an approximately 130 kDa in non-reduced SDS-PAGE. Reconstituted with sterile deionized water to 0.25 mg/mL Concentration is measured by UV.

Vascular endothelial growth factor is a key regulator of blood vessel development in embryos and angiogenesis in adult tissues. Unlike VEGF, the related VEGFC stimulates the growth of lymphatic vessels through its specificl ymphatic endothelial receptor VEGFR-3. In 1998, Dumont showed that targeted inactivation of the VEGFR-3 gene in mice resulted in defective blood vessel development in early embryos. Vasculogenesis and angiogenesis occurred, but large vessels became abnormally organized with defective lumens, leading to fluid accumulation in the pericardial cavity and cardiovascular failure at embryonic day 9.5. Thus, VEGFR3 has an essential role in the development of theembryonic cardiovascular system before the emergence of the lymphatic vessels.

Specifications

• Accession Number: P35916
• For Use With (Application): Control, ELISA Standard, Binding Assay
• Format: Lyophilized
• Formulation: PBS with 5% mannitol, 5% trehalose, 0.01% Tween 80 and no preservative; pH 7.4
• Gene ID (Entrez): 2324
• Molecular Weight (g/mol): 130 kDa
• Name: Human VEGFR3 (Flt-4) His-tag
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 100 μg
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: AI323512; Chy; chylous ascites; Flt4; FLT-4; FLT41; fms related tyrosine kinase 4; FMS-like tyrosine kinase 4; fms-related tyrosine kinase 4; LMPH1A; PCL; receptor protein tyrosine kinase; soluble VEGFR 3; sVEGFR 3; tyrosine-protein kinase receptor FLT4; Vascular endothelial growth factor receptor 3; vascular endothelial growth factor receptor-3; VEGF R3; VEGFR3; VEGFR-3
• Common Name: VEGF Receptor 3
• Gene Symbol: FLT4
• Biological Activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized human VEGF-C (at 10 μg/mL (100 μL/well) can bind human VEGFR3-his. The EC50 of human VEGFR3-his is 0.011 μg/mL)
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Human VEGFR3, amino acids Met1-Ile776 (Accession # NP_002011.2) with a C-terminal His-tag
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized human VEGF-C at 10 μg/mL (100 μL/well) can bind human VEGFR3-his. The EC50 of human VEGFR3-his is 0.011 μg/mL.
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: >97% as determined by SDS-PAGE.