Abnova™ Human ZAK (NP_598407.1, 1 a.a. - 455 a.a.) Full-length Recombinant Protein with GST-tag expressed in baculovirus infected Sf21 cells

Catalog No.	Quantity
89-967-657	5 μg

Catalog No. 89-967-657 Supplier Abnova™ Supplier No. P5667

Description

Used for Func, SDS-PAGE

This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Specifications

• Accession Number: NP_598407.1
• For Use With (Application): Functional Study, SDS-PAGE
• Formulation: Liquid
• Gene ID (Entrez): 51776
• Molecular Weight (g/mol): 79kDa
• Name: ZAK (Human) Recombinant Protein
• Preparation Method: Baculovirus infected insect cell (Sf21) expression system
• Purification Method: Glutathione sepharose chromatography
• Quality Control Testing: Loading 1 ug protein in SDS-PAGE
• Quantity: 5 μg
• Storage Requirements: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
• Regulatory Status: RUO
• Gene Alias: AZK/MLK7/MLT/MLTK/MRK/mlklak
• Common Name: ZAK
• Gene Symbol: ZAK
• Biological Activity: The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione- coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG. Substrate: MAP2K7 [inactive mutant]. ATP: 100 uM.
• Species: Baculovirus
• Recombinant: Recombinant
• Protein Tag: GST
• Expression System: Baculovirus infected insect cell (Sf21) expression system
• Form: Liquid
• Purity or Quality Grade: 67% by SDS-PAGE/CBB staining