Invitrogen™ Huntingtin Monoclonal Antibody (HDC8A4)

Catalog No.	Quantity
PIMA182100	100 μg

Catalog No. PIMA182100 Supplier Invitrogen™ Supplier No. MA182100

Description

Mouse Monoclonal Antibody

Increased cytoplasmic staining, relative to nuclear, has been reported using formaldehyde as a fixative compared with acetone/methanol. Mouse anti Huntingtin antibody, clone HDC8A4 reacts with an epitope corresponding to the HDC region (2703 - 2911 amino acids) of the huntingtin protein.

Huntingtin is a disease gene linked to Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. HD is a mid-life onset autosomal dominant neurodegenerative disease that is characterized by psychiatric disorders, dementia, and involuntary movements (chorea), leading to death in 10-20 years.The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein.

Specifications

• Antigen: Huntingtin
• Applications: Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot
• Classification: Monoclonal
• Clone: HDC8A4
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.09% sodium azide
• Gene: Htt
• Gene Accession No.: P42858, P42859
• Gene Alias: AI256365; C430023I11Rik; Hd; HD protein; HD protein homolog; Hdh; HTT; Huntingtin; huntingtin (Huntington disease); Huntingtin, myristoylated N-terminal fragment; Huntington disease gene homolog; huntington disease protein; huntington disease protein homolog; IT15; solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
• Gene Symbols: Htt
• Host Species: Mouse
• Immunogen: Recombinant protein corresponding to amino acids 2703-2911 of huntingtin.
• Purification Method: Protein G
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 100351801, 15194, 3064
• Target Species: Human, Mouse, Rabbit
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1