R&D Systems™ Hyaluronan DuoSet ELISA

Description

Includes

Kit components listed are available separately:

• PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 μm filtered
• Wash Buffer: (Catalog # WA126), or 0.05% Tween™ 20 in PBS, pH 7.2-7.4
• Reagent Diluent: (Catalog # DY004), or 5% Tween 20 in PBS, 7.2-7.4, 0.2 μm filtered
• Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
• Stop Solution: 2 N H2SO4 (Catalog # DY994) Microplates: R&D Systems (Catalog # DY990)
• Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Hyaluronan DuoSet ELISA from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural Hyaluronan. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Hyaluronan, also known as hyaluronic acid (HA) or sodium hyaluronate, is a naturally occurring linear polymer. Hyaluronan is a glycosaminoglycan (GAG) that is ubiquitously present in the extracellular matrix of all vertebrates and is also present inthe capsule of some strains of Streptococci. Mammalian hyaluronan is synthesized by one of three distinct hyaluronan synthases (HAS1, 2, and 3), which produce HA polymers with different chain lengths and differ in their rates of synthesis. Whereas high molecular weight HA (> 500 kDa) is anti-angiogenic, anti-inflammatory and immunosuppressive, low molecular weight HA (10 - 500 kDa) is highly angiogenic and pro-inflammatory. HA oligomers are anti-apoptotic and upregulate heat shock protein expression. Functionally, hyaluronan molecules are important for the maintenance of a highly hydrated extracellular matrix in tissues, which is involved in cell adhesion and supports cell migration.

The Hyaluronan DuoSet ELISA is a Solid Phase Sandwich ELISA that quantifies multi-species Hyaluronan.

Specifications

• Product Type: Solid Phase Sandwich ELISA
• Species: Human
• Binding Target: Interferon gamma
• Assay Range: 0.37 to 90 pg/mL
• Format: 96-well Microplate
• Sample Type: Cell Culture Supernatants, Serum, EDTA Plasma, Heparin Plasma
• Sample Volume: 100 μL
• Kit Contents: 96 well Micro Plates, PBS, Plate Sealers, Reagent Diluents Concentrate 2, Stop Solution, Substrate Solution, Wash Buffer
• Content And Storage: Store the unopened product at 2°C to 8°C. Do not use past expiration date.

For Research Use Only