Hygromycin B, 50 mg/ml in distilled water, sterile-filtered

Catalog No.	Quantity
AAJ673718EQ	5 x 1 mL
AAJ67371XF	1 mL

Catalog No. AAJ673718EQ Supplier Thermo Scientific Chemicals Supplier No. J673718EQ

Description

CAS: 31282-04-9 | C20H37N3O13 | 527.524 g/mol

Hygromycin B was used for hygro resistance effect in the following cells transfected 293T cells with mixture of plasmids at 160 ?g/ml, MCF-7ALOX12 cells at 150 ?g/ml, MSCV Luciferase PGK-hygro plasmid and STAT3 Reporter HeLa Stable Cell Line (stably transfected with Luciferase gene under the control of STAT3 promoter) maintained in DMEM supplemented with 10% FBS. It is suitable for the selection of cells transfected with Escherichia coli hygromycin resistance gene (hyg or hph), as well as for the maintenance of the Hygromycin phenotype of resistant cells. Consistent with its more specific effect on translocation, hygromycin B exhibits monophasic inhibition of polypeptide synthesis, suggesting that it binds to a single site on the ribosome.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Hygromycin B was used for hygro resistance effect in the following cells transfected 293T cells with mixture of plasmids at 160 μg/ml, MCF-7ALOX12 cells at 150 μg/ml, MSCV Luciferase PGK-hygro plasmid and STAT3 Reporter HeLa Stable Cell Line (stably transfected with Luciferase gene under the control of STAT3 promoter) maintained in DMEM supplemented with 10% FBS. It is suitable for the selection of cells transfected with Escherichia coli hygromycin resistance gene (hyg or hph), as well as for the maintenance of the Hygromycin phenotype of resistant cells. Consistent with its more specific effect on translocation, hygromycin B exhibits monophasic inhibition of polypeptide synthesis, suggesting that it binds to a single site on the ribosome.

Solubility
Fully miscible in water and also soluble in methanol or ethanol and buffer solution.

Notes
Protect from heat. Keep the container tightly closed and place it in a cool, dry and well ventilated condition. Store in freezer at -20°C.

Specifications

Chemical Identifiers

• CAS: 31282-04-9
• Molecular Formula: C20H37N3O13
• Molecular Weight (g/mol): 527.524
• MDL Number: MFCD06795479
• InChI Key: GRRNUXAQVGOGFE-BBMONYMYSA-N
• PubChem CID: 134129613
• IUPAC Name: (3'S,3aS,4S,4'S,5'R,6R,6'R,7R,7aS)-4-[(1R,2S,3R,5S,6S)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy-6'-[(1S)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)spiro[4,6,7,7a-tetrahydro-3aH-[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol
• SMILES: CNC1CC(C(C(C1O)OC2C3C(C(C(O2)CO)O)OC4(O3)C(C(C(C(O4)C(CO)N)O)O)O)O)N

• Type: Hygromycin B
• Physical Form: Liquid
• Concentration: 50mg/mL in distilled water
• Quantity: 5 x 1 mL
• UN Number: UN2810
• Solubility Information: Fully miscible in water and also soluble in methanol or ethanol and buffer solution.
• Chemical Name or Material: Hygromycin B, sterile-filtered

Safety and Handling

Hazard Category	H301+H331-H310-H317-H318-H334
Hazard Statement	GHS H Statement H301-H330-H334-H318-H312-H317 Toxic if swallowed. Fatal if inhaled. May cause allergy or asthma symptoms or breathing difficulties if inhaled. Causes serious eye damage. Harmful in contact with skin. May cause an allergic skin reaction.
Precautionary Statement	P261-P262-P264b-P270-P271-P272-P280-P285-P301+P310-P302+P350-P304+P340-P305+P351+P338-P310-P311-P330-P333+P313-P361-P363-P501c
DOTInformation	Transport Hazard Class: 6.1; Packing Group: III; Proper Shipping Name: TOXIC, LIQUIDS, ORGANIC, N.O.S.
EINECSNumber	250-545-5
RTECSNumber	WK2130000
TSCA	No
storageNote1	−30° to −10°C
Recommended Storage	Store at -20°C

SDS

RUO – Research Use Only