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ATCC ATCC I-13.35 (CRL-2471) mouse splenic macrophage cell line, C3H/HeJ, CSF-1 dependent
Supplier: ATCC CRL2471
ATCC I-13.35 (CRL-2471) is an immortalized mouse splenic macrophage cell line (C3H/HeJ) that exhibits constitutive antigen-presenting activity and CSF-1-dependent growth. The line is adherent and shows macrophage functions such as antibody-dependent phagocytosis, lysozyme secretion, and expression of Mac-1 and CSF-1 receptor mRNA.
- Organism: Mus musculus (mouse).
- Cell type: splenic macrophage.
- Growth characteristics: adherent; CSF-1 dependent.
- Strain and gender: C3H/HeJ, female.
- Recommended medium: DMEM with 4 mM L-glutamine, 1.5 g/L sodium bicarbonate, 4.5 g/L glucose; 70% medium, 10% fetal bovine serum, 20% LADMAC conditioned media.
- Incubation conditions: 37°C, 5% CO2.
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