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Invitrogen™ I-Block™ Protein-Based Blocking Reagent

Catalog No. T2015
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T2015 30 g
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Catalog No. T2015 Supplier Invitrogen™ Supplier No. T2015
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Description

Highly purified Casein-based blocking reagent. Thermo Scientific I-Block Protein-Based Blocking Reagent provides superior blocking compared to both dried milk and BSA.

  • Biotin-free
  • Tested in assays using Tropix substrates and alkaline phosphatase conjugates
  • Useful as blocking reagent in membrane-based and immunoassay applications
  • Suggested working
  • concentration is 0.2% (w/v) for detection of nucleic acids on neutral or positively-charged nylon membranes and for immunoassays and protein detection on membranes (nitrocellulose, PVDF, or neutral nylon)
  • Concentration of 3% is recommended for protein detection on positively-charged nylon membrane (such as TropilonPlus™ membrane)

Preparation:
Prepared in either Tris- or phosphate-buffered saline buffer with heating (40° to 50°C).

Applications:
Can be used with Western-Light™, Western-Light Plus™, Western-Star™, ELISA-Light™, Southern-Light™, and Southern-Star™ Detection Systems.

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Specifications

Chemical Name or Material Reagent
For Use With (Equipment) Western-Light™ Detection System, Western-Star™ Detection System, Southern-Star™ Detection System, ELISA-Light™ Detection System, Southern-Light™ Detection System, Western-Light Plus™ Detection System
Product Line I-Block, NovaBright
Quantity 30 g

Frequently Asked Questions (FAQs)

How can I reduce background bands in my Western blot?

Optimize the concentration of primary and secondary antibodies. In some cases, increasing the concentration of blocking agent (BSA or non-fat dry milk) or usiing an alternative blocking solution such as Starting Block or SuperBlock may reduce background signal. After incubation with the primary antibody, wash at least 2 times with TBST (include 0.5 M NaCl in one or more of the wash steps). Avoid Nonidet P40 or Triton X-100 in buffers because protein detection is decreased when these detergents are used.

For Research Use Only. Not for use in diagnostic procedures.

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