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Invitrogen™ Pichia Expression Kit, original kit

Description
The Original Pichia Expression Kit is designed for high-level expression of recombinant protein in the yeast Pichia pastoris. The vectors carry the HIS4 gene for selection of transformants on histidine-deficient medium. The Original Pichia Expression Kit provides all of the tools and reagents needed to express a gene of interest from the AOX1 promoter.

Specifications
Specifications
Antibiotic Resistance Bacterial | Ampicillin (AmpR) |
Bacterial or Yeast Strain | GS115, KM71 |
Content And Storage | The Original Pichia Expression Kit includes 10 μg each of pHIL-D2 and pPIC3.5 vectors for intracellular expression and pHIL-S1 and pPIC9 vectors for secreted expression, Pichia pastoris strains GS115 (his4), KM71(arg4 his4 aox1::ARG4), control strain GS115 Albumin, control strain GS115 β-gal, media (YP and YNB), Spheroplast Transformation Kit, and 2 μg each 5' AOX1, 3' AOX1, and α-factor sequencing primers. Store vectors and primers at -20°C. Store strains at room temperature. All components are guaranteed stable for 6 months when properly stored. |
Cloning Method | Restriction Enzyme⁄ MCS |
Cell Type | Yeast Cells |
Format | Kit |
Promoter | AOX1 |
Species | P. pastoris |
Vector | pPIC |
Expression Mechanism | Cell-Based Expression |
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For Research Use Only. Not for use in diagnostic procedures.
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