Novus Biologicals™ ICAM-3/CD50 Antibody Pair [HRP]

Description

Mouse Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human ICAM-3/CD50.

Specifications

• Assay Range: 62.5 pg/mL
• Assay Sensitivity: 62.5-4000 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human ICAM-3/CD50.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: CD50, CD50 antigen, CDW50CDw50, ICAM-3, ICAM-Rintercellular adhesion molecule-3, intercellular adhesion molecule 3
• Gene ID (Entrez): 3385
• Gene Symbol: ICAM3
• Host Species: Mouse
• Kit Contents:
  Detection Antibody: 0.5 mg/mL mouse anti-ICAM-3/CD50 monoclonal antibody conjugated to horseradish-peroxidase (HRP).
  Capture Antibody: 0.5 mg/mL of mouse anti-ICAM-3/CD50 monoclonal antibody.
  Standard: Each vial contains 40 ng of recombinant ICAM-3/CD50.
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Cellular Markers, Immunology
• Storage Requirements: Storage is content dependent.
• Target: ICAM-3/CD50
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in CBS before coating. 2. Detection Antibody: Dilute to working concentration of 1 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 4 ng/mL is recommended.