Invitrogen™ ID3 Monoclonal Antibody (2B11)

Catalog No.	Quantity
PIMA123242	100 μL

Catalog No. PIMA123242 Supplier Invitrogen™ Supplier No. MA123242

Description

Mouse Monoclonal Antibody

Recommended positive controls: Id3-transfected 293T, Id3 transfected Sol8 muscle cells and recombinant fusion protein.

Members of the Id family of basic helix-loop-helix (bHLH) proteins include ID1, ID2, ID3 and ID4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The ID-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since ID1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, the ID protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activate myogenic differentiation. It has been shown that expression of each of the ID proteins is strongly dependent on growth factor activation and that reduction of ID mRNA levels by antisense oligonucleotides leads to a delayed reentry of arrested cells into the cell cycle following growth factor stimulation.

Specifications

• Antigen: ID3
• Applications: Immunoprecipitation, Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: 2B11
• Concentration: 2.45 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with no preservative
• Gene: Id3
• Gene Accession No.: P41133, Q02535
• Gene Alias: 1R21; bHLHb25; class B basic helix-loop-helix protein 25; DNA-binding protein inhibitor ID-3; HEIR 1; HEIR1; HEIR-1; Helix-loop-helix protein HEIR-1; Hlh462; HLH642; ID 3; Id3; Id-3; Idb3; ID-like protein inhibitor HLH 1R21; ID-like protein inhibitor HLH 462; Inhibitor of differentiation 3; inhibitor of DNA binding 3; inhibitor of DNA binding 3, dominant negative helix-loop-helix protein; inhibitor of DNA binding 3, HLH protein
• Gene Symbols: Id3
• Host Species: Mouse
• Immunogen: The full-length (amino acids 1-119) mouse Id3 gene expressed in E. coli.
• Purification Method: Protein G
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 15903, 3399
• Target Species: Human, Mouse
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov