ID3 Mouse anti-Human, Clone: OTI8B3, lyophilized, TrueMAB™

Mouse Monoclonal Antibody

Supplier:  OriGene CF500713

Catalog No. 50-165-9771

Description

Reconstitute with PBS (pH 7.3) and recommend to perform another round of desalting process using Product No. 7KMWCO

Members of the Id family of basic helix-loop-helix (bHLH) proteins include ID1, ID2, ID3 and ID4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The ID-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since ID1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, the ID protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activate myogenic differentiation. It has been shown that expression of each of the ID proteins is strongly dependent on growth factor activation and that reduction of ID mRNA levels by antisense oligonucleotides leads to a delayed reentry of arrested cells into the cell cycle following growth factor stimulation.

Specifications

• Antigen: ID3
• Classification: Monoclonal
• Conjugate: Unconjugated
• Gene: ID3
• Gene Alias: HEIR-1, bHLHb25
• Host Species: Mouse
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3399
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized

• Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
• Clone: OTI8B3
• Formulation: PBS with 8% trehalose and no preservative; pH 7.3
• Gene Accession No.: Q02535
• Gene Symbols: ID3
• Immunogen: Full length human recombit protein of human ID3 produced in HEK293T cell.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1