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ATCC IDH1 Mutant U-87 Isogenic-Luc2 (HTB-14IG-LUC2) - Human glioma cell line, IDH1 R132H, luciferase (luc2) expressing
Supplier: ATCC HTB14IGLUC2
IDH1 Mutant U-87 Isogenic-Luc2 is a human glioma-derived isogenic cell line engineered to carry the IDH1 R132H mutation and constitutively express firefly luciferase (luc2). It is derived from the U-87 parental line by CRISPR gene editing and single-cell cloning, and is suitable for in vitro and in vivo bioluminescence assays to monitor tumor cell behavior and viability. Routine culture is performed in EMEM supplemented with 10% FBS and 8 μg/mL blasticidin; remove blasticidin prior to animal injection or co-culture.
- Human glioma-derived, isogenic cell line.
- Harbors IDH1 R132H (p.R132H) mutation.
- Constitutively expresses firefly luciferase (luc2) for bioluminescence assays.
- Generated by CRISPR gene editing and single-cell cloning.
- Adherent growth phenotype.
- Recommended medium: EMEM with 10% FBS and 8 μg/mL blasticidin.
- Culture conditions: 37°C, 95% air/5% CO2; subculture 1:2 to 1:6.
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