Invitrogen™ IFIT3 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA522230

Catalog No. PIPA522230

Description

Recommended positive controls: 293T. Predicted reactivity: Chimpanzee (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

IFN-induced antiviral protein which acts as an inhibitor of cellular as well as viral processes, cell migration, proliferation, signaling, and viral replication. Enhances MAVS-mediated host antiviral responses by serving as an adapter bridging TBK1 to MAVS which leads to the activation of TBK1 and phosphorylation of IRF3 and phosphorylated IRF3 translocates into nucleus to promote antiviral gene transcription. Exhibits an antiproliferative activity via the up-regulation of cell cycle negative regulators CDKN1A/p21 and CDKN1B/p27. Normally, CDKN1B/p27 turnover is regulated by COPS5, which binds CDKN1B/p27 in the nucleus and exports it to the cytoplasm for ubiquitin-dependent degradation. IFIT3 sequesters COPS5 in the cytoplasm, thereby increasing nuclear CDKN1B/p27 protein levels. Upregulates CDKN1A/p21 by downregulating MYC, a repressor of CDKN1A/p21. Can negatively regulate the apoptotic effects of IFIT2.

Specifications

• Antigen: IFIT3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: IFIT3
• Gene Alias: cig41; CIG49; CIG-49; Garg49; GARG-49; Glucocorticoid-attenuated response gene 49 protein; Ifi49; IFI60; IFI-60K; IFIT3; IFIT-3; IFIT4; IFIT-4; interferon induced protein with tetratricopeptide repeats 3; interferon-induced 60 kDa protein; Interferon-induced protein with tetratricopeptide repeats 3; interferon-induced protein with tetratricopeptide repeats 4; interferon-stimulated protein 60; IRG2; Isg49; ISG60; ISG-60; P49; P60; retinoic acid-induced gene G protein; RIG-G; RP11-149I23.4
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 15959, 3437
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.3 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: O14879, Q64345
• Gene Symbols: IFIT3
• Immunogen: Recombinant fragment corresponding to a region within amino acids 230 and 490 of IFIT3 (Uniprot ID#O14879).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG