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Invitrogen™ IFN gamma Monoclonal Antibody (2G1)
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Catalog No. ENM700A
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500 μg
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ENM700A 500 μg
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Catalog No. ENM700A Supplier Invitrogen™ Supplier No. M700A
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Description

Mouse Monoclonal Antibody

M700A targets IFN gamma in ELISA, and Western blot applications and shows reactivity with Human samples. The M700A anti-IFN Gamma antibody (clone 2G1) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M701B (biotinylated conjugate of clone B133.5). Typical dilutions for sandwich ELISA include 1 μg/mL for coating and 0.125-0.25 μg/mL for detection. The M700A immunogen is recombinant human IFN gamma. M700A detects IFN gamma which has a predicted molecular weight of approximately 16 kDa. The M700A IFNg antibody (clone 2G1) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M701B (biotinylated conjugate of clone B133.5). Typical dilutions for sandwich ELISA include 1 μg/mL for coating and 0.125 - 0.25 μg/mL for detection. This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg). Antibody M700A (clone 2G1) and biotinylated antibody M701B (clone B133.5) have successfully been used in combination with recombinant IFNg protein SIFNG in ELISA applications.

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

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Specifications

Antigen IFN gamma
Applications ELISA, Western Blot
Classification Monoclonal
Clone 2G1
Concentration 1.0 mg/mL
Conjugate Unconjugated
Formulation PBS with no preservative
Gene IFNG
Gene Accession No. P01579
Gene Alias BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN γ; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFNγ; Immune interferon; Interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon γ; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferonγ; M-IFN-g; R-IFN-g
Gene Symbols IFNG
Host Species Mouse
Immunogen Recombinant human IFN gamma.
Purification Method Protein G
Quantity 500 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 3458
Target Species Human
Content And Storage -20°C
Product Type Antibody
Form Liquid
Isotype IgG1 κ
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