Invitrogen™ IFN gamma Monoclonal Antibody (XMG1.2)

Rat Monoclonal Antibody

Supplier:  Invitrogen™ MM700

Catalog No. ENMM700

Description

MM700 targets IFN gamma in ELISA, IHC(F), Neu, and WB applications and shows reactivity with mouse samples. The MM700 immunogen is recombinant mouse IFN gamma. MM700 detects IFN gamma which has a predicted molecular weight of approximately 16 kDa. The MM700 mouse IFNg antibody (clone XMG1.2) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody MM700B (biotinylated conjugate of the same clone) Typical dilutions for sandwich ELISA include 1 μg/mL for coating and 0.125 - 0.25 μg/mL for detection. Antibody MM700 (clone XMG1.2) and biotinylated antibody MM700B (same clone) have successfully been used in combination with recombinant mouse IFNg protein SM2IFNG in ELISA applications. This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified. This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

Specifications

• Antigen: IFN gamma
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P01580
• Gene Symbols: IFNG
• Immunogen: Recombinant mouse IFN gamma.
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Immunohistochemistry (Frozen), Neutralization, Western Blot
• Clone: XMG1.2
• Conjugate: Unconjugated
• Gene: IFNG
• Gene Alias: BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN ?; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFN?; Immune interferon; Interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon ?; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferon?; M-IFN-g; R-IFN-g
• Host Species: Rat
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 15978
• Content And Storage: -20°C
• Form: Liquid
• Green Features: Responsibly packaged