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  6. Invitrogen™ IFN gamma Monoclonal Antibody (XMG1.2), Functional Grade, eBioscience™, Invitrogen™ 

Invitrogen™ IFN gamma Monoclonal Antibody (XMG1.2), Functional Grade, eBioscience™, Invitrogen™
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Catalog No. p-7069930
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50-147-40 50 μg
50-147-41 500 μg
16-731-138 5 mg
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Catalog No. 50-147-40 Supplier Invitrogen™ Supplier No. 16731181
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Description

Rat Monoclonal Antibody

Description: The XMG1.2 antibody reacts with mouse interferon (IFN) gamma. The XMG1.2 antibody is a neutralizing antibody. Mouse IFN gamma is a 20 kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN gamma, in synergy with other cytokines such as TNF alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN gamma. Glycosylation of IFN gamma does not affect its biological activity. Applications Tested: The Functional Grade Purified XMG1.2 antibody has been tested by LAL assay to verify low endotoxin levels and has been tested for ELISA capture and in bioassay for neutralization of IFN gamma bioactivity. The XMG1.2 antibody at 15 ng/mL has been found to inhibit by 50% the biological effects of 1 ng/mL of mouse IFN gamma (ND50), in an EMCV assay of L929 cell protection. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols™ section. The XMG1.2 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse IFN gamma in combination with the biotin R4-6A2 (13-7312) antibody for detection and recombit mouse IFN gamma (39-8311) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 0.25 - 1 μg/mL.

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.
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Specifications

Antigen IFN gamma
Applications Functional Assay, Neutralization
Classification Monoclonal
Clone XMG1.2
Concentration 1 mg/mL
Conjugate Functional Grade
Formulation PBS with no preservative; pH 7.2
Gene IFNG
Gene Accession No. P01580
Gene Alias BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN γ; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFNγ; Immune interferon; Interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon γ; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferonγ; M-IFN-g; R-IFN-g
Gene Symbols IFNG
Host Species Rat
Purification Method Affinity chromatography
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 15978
Target Species Mouse
Content And Storage 4°C
Product Type Antibody
Form Liquid
Isotype IgG1 κ
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Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.

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