Invitrogen™ IFNAR2 Monoclonal Antibody (MMHAR-2)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 213851

Catalog No. PI213851

Description

This antibody neutralizes human IFN-alpha receptor, interacting with the extracellular domain. It binds to human IFN-alpha receptor with high affinity and blocks biological action of Type I IFNs. To measure bioactivity, interferon was titrated with the use of a cytopathic effect inhibition assay, where one neutralization unit is the amount of antiserum required to neutralize one unit of human interferon alpha to a 50% endpoint. In this antiviral assay, approximately one unit/mL of interferon was necessary to produce a cytopathic effect of 50%. Purity is >95% by SDS-PAGE and endotoxin level <1 EU/ug. Store at -20°C for up to 6 months. For long-term storage, store at -70°C for retention of full activity. Thaw product by incubation in cold water and aliquot into separate vials, adding 0.1% BSA for additional stability. Refreeze product using a dry ice or a dry ice/alcohol bath. Packaged by and distributed for PBL Assay Science.

The type I interferons, IFN-alpha and IFN-beta, are a group of structurally and functionally related proteins that are induced by either viruses or double-stranded RNA and are defined by their ability to confer an antiviral state in cells. IFN-alpha and IFN-beta appear to compete with one another for binding to a common cell surface receptor, while immune IFN (IFN-gamma) binds to a distinct receptor. This distinct receptor, IFN-alphaR, is only weakly responsive to type I interferons, in contrast to IFN-alpha/betaR, which binds to and responds effectively to IFN-beta and to several of the IFN-alpha subtypes. IFN-alpha/betaR is expressed as two alternatively spliced transcripts, designated IFN-alpha/betaRalpha (IFN-alpha/betaR1) and IFN-alpha/betaRbeta (IFN-alpha/betaR2), both of which are involved in signal transduction and ligand binding.

Specifications

• Antigen: IFNAR2
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.1% BSA and no preservative
• Gene Accession No.: P48551
• Gene Symbols: IFNAR2
• Immunogen: Human interferon alpha/beta receptor chain 2.
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Functional Assay, Neutralization
• Clone: MMHAR-2
• Conjugate: Unconjugated
• Gene: IFNAR2
• Gene Alias: AI747302; human interferon alpha/beta receptor; IFN alpha/betaRbeta; IFN R2; IFNABR; IFNalpha/beta R2; IFN-alpha/beta receptor 2; IFNalpha/betaR2; IFN-alpha/betaR2; IFN-alpha/betaRbeta; IFNalpha/beta-Rbeta; IFN-alpha-REC; IFNAR2; Ifnar-2; IFNARB; IFN-R; IFNR2; IFN-R-2; IMD45; interferon (alpha and beta) receptor 2; interferon (alpha, beta and omega) receptor 2; interferon alpha and beta receptor subunit 2; interferon alpha binding protein; Interferon alpha/beta receptor 2; interferon receptor 2c; interferon-alpha/beta receptor 2; interferon-alpha/beta receptor beta chain; type I interferon alpha/beta receptor 2a; type I interferon receptor 2
• Host Species: Mouse
• Purification Method: Ion-exchange chromatography, Size-exclusion chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3455
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid
• Green Features: Responsibly packaged