Invitrogen™ IgD Monoclonal Antibody (10.4.22), Functional Grade, eBioscience™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 16592485

Catalog No. 50-112-8593

Description

Description: This 10.4.22 monoclonal antibody reacts with the a allotype of mouse IgD, which is expressed by mature peripheral B cells. This antibody has been reported to activate B cells via engagement of the IgD receptor. The use of anti-IgM as a polyclonal B cell activator is problematic due to interference from high levels of circulating serum IgM. Crossblocking studies suggest that the 10.4.22 antibody recognizes a different epitope from 11-26c. Applications Reported: This 10.4.22 antibody has been reported for use in functional assays. Applications Tested: This 10.4.22 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.

Specifications

• Antigen: IgD
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: P01881
• Gene Symbols: Ighd
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 380797
• Content And Storage: 4°C
• Form: Liquid

• Applications: Functional Assay
• Clone: 10.4.22
• Conjugate: Functional Grade
• Gene: Ighd
• Gene Alias: IgD; Igh-5; IGHD; immunoglobulin heavy constant delta
• Host Species: Mouse
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ