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  6. Invitrogen™ Mouse IgD Monoclonal Antibody (11-26c (11-26)), Super Bright™ 436, eBioscience™ 

Invitrogen™ Mouse IgD Monoclonal Antibody (11-26c (11-26)), Super Bright™ 436, eBioscience™
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Catalog No. 62599382
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62-599-382 100 μg
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Catalog No. 62-599-382 Supplier Invitrogen™ Supplier No. 62599382
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Description

Rat Monoclonal Antibody

Description: The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM. IgD is expressed by peripheral mature B cells. 11-26c does not activate B cells. Applications Reported: This 11-26 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 11-26 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickin...

This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.
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Specifications

Antigen Mouse IgD
Applications Flow Cytometry
Classification Monoclonal
Clone 11-26c (11-26)
Concentration 0.2 mg/mL
Conjugate Super Bright 436
Formulation PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Ighd
Gene Accession No. P01881
Gene Alias IgD; Igh-5; IGHD; immunoglobulin heavy constant delta
Gene Symbols Ighd
Host Species Rat
Purification Method Affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 380797
Target Species Mouse
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG2a κ
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Safety and Handling

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