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  6. Invitrogen™ Human IgD Monoclonal Antibody (IA6-2), NovaFluor™ Yellow 810, eBioscience™ 

Invitrogen™ Human IgD Monoclonal Antibody (IA6-2), NovaFluor™ Yellow 810, eBioscience™

Catalog No. H026T03Y09A
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Description

Mouse Monoclonal Antibody

This IA6-2 monoclonal antibody reacts with the heavy chain of human immunoglobulin D (IgD). This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding. This product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer. Applications Tested This IA6-2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.2 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Master mixes Master mixes of NFs should be made at 2-8 °C and may be made up to 4 hours ahead of time. We do not recommend storing master mixes containing NovaFluor conjugates overnight or longer. Whole Blood compatibility When utilizing whole blood (as opposed to density-gradient-purified PBMC), we recommend lysing red blood cells in bulk prior to staining with NovaFluor conjugates. See the Bulk Lysis of Human Whole Blood protocol here. Staining of whole blood with NovaFluor conjugates followed by lysis...

This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Human IgD
Applications Flow Cytometry
Classification Monoclonal
Clone IA6-2
Concentration 0.2 μg/Test
Conjugate NovaFluor Yellow 810
Formulation PBS with BSA and 0.09% sodium azide; pH 7.2
Gene IGHD
Gene Accession No. P01880
Gene Alias Ig delta chain constant region; IGHD; IGHD1; IGHDELTA; immunoglobulin heavy constant delta
Gene Symbols IGHD
Host Species Mouse
Purification Method Affinity chromatography
Quantity 100 Tests
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 3495
Target Species Human
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG2a κ
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