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Invitrogen™ IgE Monoclonal Antibody (23G3), Biotin, eBioscience™, Invitrogen™

Catalog No. 5012108
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50-121-08 50 μg
50-121-09 100 μg
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Catalog No. 50-121-08 Supplier Invitrogen™ Supplier No. 13599281
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Description

Rat Monoclonal Antibody

Description: The 23G3 monoclonal antibody reacts with the epsilon heavy chain of mouse IgE. It does not react with other classes of mouse immunoglobulin including IgD, IgG, IgA, or IgM. Applications Reported: The 23G3 antibody has been reported for use in flow cytometric analysis, and ELISA. Applications Tested: The 23G3 antibody has been tested by ELISA and can be used at 1:5000-1:10000. This 23G3 antibody has also been tested by flow cytometric analysis of IGEL cells, and can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.

IgE is one of the five classes of immunoglobulins found in mammals. Monomers consist of two heavy (epsilon) chains with 4 Ig-like constant domains, and two light chains. IgE plays an essential role in type I hypersensitivity (allergic responses such as hay fever, asthma, hives, anaphylatic shock) by binding to Fc receptors on basophils and mast cells. Crosslinking of IgE bound to Fc receptors induces degranulation of mast cells and basophils that promote allergic manifestations. IgE is also functionally important for immunity against parasites.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Mouse IgE
Applications ELISA, Flow Cytometry
Classification Monoclonal
Clone 23G3
Concentration 0.5 mg/mL
Conjugate Biotin
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene Ighe
Gene Accession No. P06336
Gene Alias Gm900; Ighe; Immunoglobulin E; Immunoglobulin heavy constant epsilon; ImmunoglobulinE
Gene Symbols Ighe
Host Species Rat
Purification Method Affinity chromatography
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 380792
Target Species Mouse
Content And Storage 4°C, do not freeze
Product Type Antibody
Form Liquid
Isotype IgG1 κ
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For Research Use Only.

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