Invitrogen™ Human IgE Monoclonal Antibody (Ige21), PE-Cyanine7, eBioscience™

Catalog No.	Quantity
25-698-642	100 Tests

Catalog No. 25-698-642 Supplier Invitrogen™ Supplier No. 25698642

Description

Mouse Monoclonal Antibody

Description: The monoclonal antibody IgE21 recognizes the immunoglobulin E class of antibodies. The natural levels of IgE in the serum are quite low, comprising only a small portion of the total immunoglobulin. IgE plays a role in allergy responses by binding to mast cells and basophils through the Fc epsilon receptor I which results in the release of histamine and other molecules. The IgE is produced by terminally differentiated plasma B cells and basophils. Like all immunoglobulins, IgE is expressed as a soluble molecule as well as a membrane bound form. IgE21 recognizes both forms. Applications Reported: This Ige21 antibody has been reported for use in flow cytometric analysis. Applications Tested: This Ige21 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficie...

IgE is one of the five classes of immunoglobulins found in mammals, characterized by its monomeric structure consisting of two heavy (epsilon) chains with four Ig-like constant domains and two light chains. Although IgE comprises only a small portion of the total immunoglobulin in serum, it plays a crucial role in type I hypersensitivity reactions, such as hay fever, asthma, hives, and anaphylactic shock. IgE is produced by terminally differentiated plasma B cells and basophils. It binds to Fc epsilon receptors on mast cells and basophils, leading to the release of histamine and other molecules that promote allergic responses. Crosslinking of IgE bound to these receptors induces degranulation of mast cells and basophils, resulting in allergic manifestations. In addition to its role in allergic responses, IgE is functionally important for immunity against parasites, highlighting its dual role in both protective and pathological immune processes.

Specifications

• Antigen: Human IgE
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: Ige21
• Concentration: 5 μL/Test
• Conjugate: PE-Cyanine7
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene: IGHE
• Gene Accession No.: P01854
• Gene Alias: HCAK1; IgA1; IgG3; IGHA1; IGHA2; IGHG1; IGHG2; IGHG3; IGHG4; IGKC; IGKCD; IGLC; IGLC1; Immunoglobulin E; immunoglobulin heavy constant alpha 1; immunoglobulin heavy constant alpha 2 (A2m marker); immunoglobulin heavy constant gamma 1 (G1m marker); immunoglobulin heavy constant gamma 2 (G2m marker); immunoglobulin heavy constant gamma 3 (G3m marker); immunoglobulin heavy constant gamma 4 (G4m marker); immunoglobulin kappa constant; immunoglobulin lambda constant 1; ImmunoglobulinE; Km
• Gene Symbols: IGHE
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 100 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 3497
• Target Species: Human
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ