Invitrogen™ IGF1R (CD221) Monoclonal Antibody (1H7)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA181739

Catalog No. MA181739

Description

Clone 1H7 recognizes an epitope in the alpha subunits of CD221, demonstrated by Western blotting, and is reported to partically block binding of IGF-I and IGF-II to CD221. This product requires protein digestion pre-treatment of paraffin sections using trypsin or pronase. A suggested positive control for immunohistochemical applications is pancreas or placenta. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. This product is a low-endotoxin, preservative-free formulation. Endotoxin level is <0.01 EU/ug.

IGF1R, also known as Insulin-like Growth Factor 1 (IGF-1) Receptor, is a receptor tyrosine kinase activated by IGF-1 and IGF-2. IGFR has been implicated in a variety of cancers. The protein is a tetramer consisting of two alpha, and two beta subunits. The alpha-subunits binds to insulin growth factor 1 with higher affinity than to insulin growth factor 2. Binding results in a conformational change, followed by autophosphorylation of IGF-1R at positions 1134, 1135, 1136. The activated IGF-1R in turn, causes phosphorylation of substrate proteins, followed by sequential activation of RAS, RAF, and mitogen-activated protein kinase isoforms ERK, p38, and JNK, leading to the transcription of genes that drive proliferation.

Specifications

• Antigen: IGF1R (CD221)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.4
• Gene Accession No.: P08069
• Gene Symbols: IGF1R
• Immunogen: Purified human placental IGF-1 receptor.
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry (Paraffin), Western Blot
• Clone: 1H7
• Conjugate: Unconjugated
• Gene: IGF1R
• Gene Alias: A330103N21Rik; CD221; CD221 antigen; D930020L01; hyft; IGF 1 receptor; IGF 1R; IGF1R; IGF-1R; IGF-I receptor; IGFIR; IGFIRC; IGFR; Igfr1; insulin like growth factor 1 receptor; Insulin like growth factor 1 receptor precursor; insulin-like growth factor 1 receptor; Insulin-like growth factor 1 receptor alpha chain; Insulin-like growth factor 1 receptor beta chain; Insulin-like growth factor I receptor; JTK13; line 186; soluble IGF1R variant 1; soluble IGF1R variant 2
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 3480
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov