Invitrogen™ IGF1R (CD221) Monoclonal Antibody (1H7)

Catalog No.	Quantity
MA181739	500 μg

Catalog No. MA181739 Supplier Invitrogen™ Supplier No. MA181739

Description

Mouse Monoclonal Antibody

Clone 1H7 recognizes an epitope in the alpha subunits of CD221, demonstrated by Western blotting, and is reported to partically block binding of IGF-I and IGF-II to CD221. This product requires protein digestion pre-treatment of paraffin sections using trypsin or pronase. A suggested positive control for immunohistochemical applications is pancreas or placenta. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. This product is a low-endotoxin, preservative-free formulation. Endotoxin level is <0.01 EU/ug.

IGF1R, also known as Insulin-like Growth Factor 1 (IGF-1) Receptor, is a receptor tyrosine kinase activated by IGF-1 and IGF-2. IGFR has been implicated in a variety of cancers. The protein is a tetramer consisting of two alpha, and two beta subunits. The alpha-subunits binds to insulin growth factor 1 with higher affinity than to insulin growth factor 2. Binding results in a conformational change, followed by autophosphorylation of IGF-1R at positions 1134, 1135, 1136. The activated IGF-1R in turn, causes phosphorylation of substrate proteins, followed by sequential activation of RAS, RAF, and mitogen-activated protein kinase isoforms ERK, p38, and JNK, leading to the transcription of genes that drive proliferation.

Specifications

• Antigen: IGF1R (CD221)
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry (Paraffin), Western Blot
• Classification: Monoclonal
• Clone: 1H7
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with no preservative; pH 7.4
• Gene: IGF1R
• Gene Accession No.: P08069
• Gene Alias: A330103N21Rik; CD221; CD221 antigen; D930020L01; hyft; IGF 1 receptor; IGF 1R; IGF1R; IGF-1R; IGF-I receptor; IGFIR; IGFIRC; IGFR; Igfr1; insulin like growth factor 1 receptor; Insulin like growth factor 1 receptor precursor; insulin-like growth factor 1 receptor; Insulin-like growth factor 1 receptor alpha chain; Insulin-like growth factor 1 receptor beta chain; Insulin-like growth factor I receptor; JTK13; line 186; soluble IGF1R variant 1; soluble IGF1R variant 2
• Gene Symbols: IGF1R
• Host Species: Mouse
• Immunogen: Purified human placental IGF-1 receptor.
• Purification Method: Protein A
• Quantity: 500 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 3480
• Target Species: Human
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov