Novus Biologicals™ IGFBP-6 Antibody Pair [HRP]

Description

Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human IGFBP-6.

Specifications

• Assay Range: 7.8 pg/mL
• Assay Sensitivity: 7.8-500 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human IGFBP-6.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: IBP6, IBP-6, IGF binding protein 6, IGF-binding protein 6, IGFBP-6, insulin-like growth factor binding protein 6, insulin-like growth factor-binding protein 6
• Gene ID (Entrez): 3489
• Gene Symbol: IGFBP6
• Host Species: Rabbit/Mouse
• Kit Contents:
  Detection Antibody: 0.2 mg/mL of rabbit anti-Human IGFBP-6 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  Capture Antibody: 0.5 mg/mL of mouse anti-Human IGFBP-6 monoclonal antibody (in PBS, pH 7.4).
  Standard: Each vial contains 45 ng of recombinant Human IGFBP-6.
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Cell Cycle and Replication
• Storage Requirements: Storage is content dependent.
• Target: IGFBP-6
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 500 pg/mL is recommended.