IgG Fc Sheep anti-Guinea Pig, Biotin, Polyclonal, Secondary Antibody, Novus Biologicals™

Sheep Polyclonal Antibody

Supplier:  Novus Biologicals NBP269478

Catalog No. NBP2-69478

Description

IgG Fc Polyclonal antibody specifically detects IgG Fc in Guinea Pig samples. It is validated for ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen).

Specifications

• Antigen: IgG Fc
• Classification: Polyclonal
• Conjugate: Biotin
• Formulation: Lyophilized from PBS (pH 7.2) with No Preservative
• Immunogen: Purified normal IgG isolated from pooled guinea pig serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
• Quantity: 1 mL
• Test Specificity: The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule which expresses strict isotypic (class) specificity. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal guinea pig plasma and serum, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG. It does not react with IgM, IgA and IgG/Fab or any non-Ig protein in guinea pig serum.
• Target Species: Guinea Pig
• Form: Lyophilized

• Applications: ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen)
• Concentration: LYOPH
• Dilution: ELISA 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunohistochemistry-Frozen 1:10 - 1:500
• Host Species: Sheep
• Purification Method: Ion exchange chromatography
• Primary or Secondary: Secondary
• Reconstitution: Reconstitute by by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C.
• Content And Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.