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IgG Fc Sheep anti-Guinea Pig, Biotin, Polyclonal, Secondary Antibody, Novus Biologicals™
SDP

Catalog No. NBP269478 Shop All R&D Systems Products
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NBP2-69478 1 mL
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Catalog No. NBP2-69478 Supplier Novus Biologicals Supplier No. NBP269478

Description

Sheep Polyclonal Antibody

IgG Fc Polyclonal antibody specifically detects IgG Fc in Guinea Pig samples. It is validated for ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen).

Specifications

Antigen IgG Fc
Applications ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen)
Classification Polyclonal
Concentration LYOPH
Conjugate Biotin
Dilution ELISA 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunohistochemistry-Frozen 1:10 - 1:500
Formulation Lyophilized from PBS (pH 7.2) with No Preservative
Host Species Sheep
Immunogen Purified normal IgG isolated from pooled guinea pig serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Purification Method Ion exchange chromatography
Quantity 1 mL
Primary or Secondary Secondary
Test Specificity The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule which expresses strict isotypic (class) specificity. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal guinea pig plasma and serum, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG. It does not react with IgM, IgA and IgG/Fab or any non-Ig protein in guinea pig serum.
Reconstitution Reconstitute by by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C.
Target Species Guinea Pig
Content And Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Form Lyophilized
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