Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™, Invitrogen™

Mouse Isotype Control

Catalog Number	Mfr. No.	Quantity
50-155-69	Invitrogen 25471480	25 μg
50-155-67	Invitrogen 25471441	25 Tests
50-155-68	Invitrogen 25471442	100 Tests

Description

Description: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control. Applications Reported: PE-Cy7 Mouse IgG1, K Iso Cntrl has been reported for use in flow cytometric analysis. Applications Tested: This P3.6.2.8.1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at the same concentration as the experimental antibody. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

Mouse IgG1 is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG1 is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG1 is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.

Specifications

• Antigen: Mouse IgG1 kappa
• Applications: Control, Flow Cytometry
• Form: Liquid
• Regulatory Status: RUO
• Gene Alias: IgG1; Igh-4; Ighg1; Immunoglobulin G; Immunoglobulin G1; immunoglobulin heavy constant gamma 1 (G1m marker); ImmunoglobulinG; ImmunoglobulinG1; VH7183
• Primary or Secondary: Isotype Control (Primary)
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!

• Clone: P3.6.2.8.1
• Conjugate: PE-Cyanine7
• Host Species: Mouse
• Target Species: Not Applicable
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography

For Research Use Only.