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Mouse IgG2a kappa, Functional Grade, clone: eBM2a, Isotype Control, eBioscience™ Non-distribution product as customer accommodation.

Mouse Isotype Control

$63.00 - $195.00

Specifications

Antigen Mouse IgG2a kappa
Clone eBM2a
Target Species Not Applicable
Conjugate Functional Grade
Isotype IgG2a, kappa
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Products 3
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-145-35 Non-distribution product as customer accommodation.
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Invitrogen
16472481
50μg
Each for $63.00
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50-145-36 Non-distribution product as customer accommodation.
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Invitrogen
16472482
100μg
Each for $108.00
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50-145-37 Non-distribution product as customer accommodation.
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Invitrogen
16472485
500μg
Each for $195.00
Add to cart
 
Description

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Description: This is a monoclonal mouse IgG2a, kappa antibody. It is used as an isotype control for mouse IgG2a antibodies.Applications Reported: This mouse IgG2a isotype control has been reported for use in bioassays and cell surface and intracellular flow cytometric analysis.Applications Tested: This mouse IgG2a isotype control has been tested by flow cytometric analysis of normal human peripheral blood cells. It should be used at the same concentration as the experimental antibody. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

Mouse IgG2a kappa
Not Applicable
IgG2a, kappa
Monoclonal
Control
PBS with no preservative; pH 7.2
RUO
eBM2a
Functional Grade
Control, Flow Cytometry, Functional Assay
Liquid
Isotype Control
4° C
SDS

For Research Use Only.

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