Novus Biologicals™ IkB-alpha Antibody Pair [HRP]

Catalog No.	Quantity
NB009805	15 Plates

Catalog No. NB009805 Supplier Novus Biologicals™ Supplier No. NBP27938315PLATES

Description

Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human IkB-alpha.

Specifications

• Assay Range: 12.5 pg/mL
• Assay Sensitivity: 12.5-800 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human IkB-alpha.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: alpha, I-kappa-B-alpha, IkappaBalphaNF-kappa-B inhibitor alpha, IKBAikB-alpha, IkB-alpha, MAD3, MAD-3, Major histocompatibility complex enhancer-binding protein MAD3, NFKBI, nuclear factor of kappa light chain gene enhancer in B-cells, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor
• Gene ID (Entrez): 4792
• Gene Symbol: NFKBIA
• Host Species: Mouse/Rabbit
• Kit Contents:
  Detection Antibody: 0.5 mg/mL mouse anti-IkB-alpha monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % glycerol, pH 7.4).
  Capture Antibody: 1.0 mg/mL of rabbit anti-IkB-alpha monoclonal antibody (in PBS, pH 7.4).
  Standard: Each vial contains 22 ng of recombinant IkB-alpha.
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Apoptosis, Cell Biology, Chaperone Mediated Autophagy (CMA), Diabetes Research, DNA Repair, Nucleotide Excision Repair, Phospho Specific, Signal Transduction
• Storage Requirements: Storage is content dependent.
• Target: IkB-alpha
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in CBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 0.8 ng/mL is recommended.