Novus Biologicals™ IL-1 RII Antibody Pair [HRP]

Catalog No.	Quantity
NB010317	15 Plates

Catalog No. NB010317 Supplier Novus Biologicals™ Supplier No. NBP27963915PLATES

Description

Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Rat IL-1 RII.

Specifications

• Assay Range: 12.5 pg/mL
• Assay Sensitivity: 12.5-800 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Rat IL-1 RII.
• Target Species: Rat
• For Use With (Application): Sandwich ELISA
• Gene Alias: beta, CD121b, CD121b antigen, IL1RBCD121 antigen-like family member B, IL-1R-beta, IL-1RT2, IL-1RT-2, interleukin 1 receptor, type II, Interleukin-1 receptor beta, MGC47725
• Gene ID (Entrez): 7850
• Gene Symbol: IL1R2
• Host Species: Mouse/Rabbit
• Kit Contents:
  Detection Antibody: 0.2 mg/mL of mouse anti-IL-1 RII monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  Capture Antibody: 1 mg/mL of rabbit anti-Rat IL-1 RII monoclonal antibody (in PBS, pH 7.4).
  Standard: Each vial contains 45 ng of recombinant Rat IL-1 RII.
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Cancer, Phospho Specific, Signal Transduction
• Storage Requirements: Storage is content dependent.
• Target: IL-1 RII
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 800 pg/mL is recommended.