Novus Biologicals™ IL-10 Antibody Pair [HRP]

Description

Polyclonal/Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes an antibody specific for Human IL-10.

Specifications

• Assay Range: 14.06 pg/mL
• Assay Sensitivity: 14.06-900 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes an antibody specific for Human IL-10.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: CSIFMGC126450, Cytokine synthesis inhibitory factor, IL10A, IL-10MGC126451, interleukin 10, interleukin-10, TGIF
• Gene ID (Entrez): 3586
• Gene Symbol: IL10
• Host Species: Rabbit/Mouse
• Kit Contents:
  Detection Antibody: 0.2 mg/mL of rabbit anti-Human IL-10 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  Capture Antibody: 1 mg/mL of mouse anti-Human IL-10 monoclonal antibody (in PBS, pH 7.4).
  Standard: Each vial contains 55 ng of recombinant Human IL-10.
• Quantity: 5 Plates
• Regulatory Status: RUO
• Research Discipline: Adaptive Immunity, Apoptosis, Asthma, B Cell Development and Differentiation Markers, Biologically Active Proteins, Cancer, Cytokine Research, Immunology, Innate Immunity
• Storage Requirements: Storage is content dependent.
• Target: IL-10
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 900 pg/mL is recommended.