Invitrogen™ IL-10 Monoclonal Antibody (JES3-9D7), Brilliant Ultra Violet™ 395, eBioscience™

Catalog No.	Quantity
36-371-0842	100 Tests
36-371-0841	25 Tests

Catalog No. 36-371-0842 Supplier Invitrogen™ Supplier No. 363710842

Description

Rat Monoclonal Antibody

Description The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10). Applications Tested This JES3-9D7 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to 'Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins' located at Flow Protocols . This may be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Blocking Buffers When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401 ) or Brilliant Stain Buffer (Product # 00-4409-75 ) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information. Fixation Samples can be stored in IC Fixation Buffer (Product # 00-8222 ) (100 ...

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

Specifications

• Antigen: IL-10
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: JES3-9D7
• Concentration: 5 μL/Test
• Conjugate: Brilliant Ultraviolet 395
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene: IL10
• Gene Accession No.: P22301
• Gene Alias: CSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF
• Gene Symbols: IL10
• Host Species: Rat
• Purification Method: Affinity chromatography
• Quantity: 100 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 3586
• Target Species: Human
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ