Invitrogen™ IL-12/IL-23 p40 Monoclonal Antibody (C17.8), Functional Grade, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  Invitrogen™ 16712381

Catalog No. 50-147-18

Description

Description: The C17.8 antibody reacts with the p40 subunit of IL-12 and IL-23. The C17.8 antibody has been found to neutralize IL-12 and IL-23 bioactivity. For specific neutralization of IL-23 only, please see clone G23-8 (anti-mouse IL-23 p19). IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted by activated monocytes, macrophages, and dendritic cells. IL-23 is a heterodimer composed of the disulfide-linked p19 protein and p40 protein, expressed by activated dendritic cells. Applications Reported: The C17.8 antibody has been reported for use in IP, Western, ELISA, neutralization, and intracellular staining for flow cytometric analysis. Applications Tested: The Functional Grade Purified C17.8 antibody has been tested by LAL assay to verify low endotoxin levels and has been tested for ELISA detection and in bioassay for neutralization of IL-12 p70 and IL-23 bioactivity. The C17.8 antibody at 62.0 ng/mL has been found to inhibit by 50% the biological effects of 1 ng/mL mouse IL-12 p70 (14-8121) or mouse IL-23 (14-8231), in corresponding bioassay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols™ section. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered.

IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.

Specifications

• Antigen: IL-12/IL-23 p40
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: P43431, P43432, Q9EQ14
• Gene Symbols: IL12A, IL12B, Il23a
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16159, 16160, 83430
• Content And Storage: 4°C
• Form: Liquid

• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Neutralization, Western Blot
• Clone: C17.8
• Conjugate: Functional Grade
• Gene: IL12A
• Gene Alias: CLMF; CLMF p35; CLMF p40; CLMF2; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12/IL 23p40; il 23; IL 23 p40; Il12; Il-12 p35; IL12 p40; IL23; IL-12 subunit p35; IL-12 subunit p40; IL-12, subunit p35; IL12, subunit p40; Il12a; IL-12A; Il12b; IL-12B; IL12p35; IL-12p35; Il12p40; Il-12p40; il23; IL-23; IL23 p41; IL-23 subunit alpha; Il23a; IL-23A; IL-23-A; IL23P19; IL-23p19; IL35 subunit; ILN; IMD28; IMD29; Interleukin; Interleukin 12; interleukin 12 35 kDa subunit; interleukin 12 35kDa subunit; interleukin 12 40 kDa subunit; interleukin 12 40kDa subunit; interleukin 12 p35 subunit; interleukin 12 polypeptide 35; interleukin 12 subunit p40; interleukin 12, p35; interleukin 12, p40; interleukin 12A; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin 12b; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin 23 p19 subunit; interleukin 23 subunit alpha; interleukin 23, alpha subunit p19; Interleukin12; interleukin-12 alpha chain; interleukin-12 beta chain; interleukin-12 p40; interleukin-12 p40 subunit; interleukin-12 subunit alpha; Interleukin-12 subunit beta; interleukin-12 subunit p40; interleukin-23 p19 subunit; interleukin-23 p40 subunit; interleukin-23 subunit alpha; Interleukin-23 subunit p19; interleukin-six, G-CSF related factor; JKA3 induced upon T-cell activation; Ll12a; natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35; natural killer cell stimulatory factor, 40 kD subunit; NF cell stimulatory factor chain 1; NFSK; NK cell stimulatory factor chain 1; NK cell stimulatory factor chain 2; NKSF; NKSF1; NKSF2; p19; P35; p40; RP23-388G23.1; SGRF; UNQ2498/PRO5798
• Host Species: Rat
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ

For Research Use Only.