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Invitrogen™ IL-12/IL-23 p40 Monoclonal Antibody (C17.8), PE-Cyanine7, eBioscience™
Rat Monoclonal Antibody
Supplier: Invitrogen™ 25712382

Description
Description: The C17.8 antibody reacts with the p40 subunit of IL-12 and IL-23. The C17.8 antibody has been found to neutralize IL-12 and IL-23 bioactivity. For specific neutralization of IL-23 only, please see clone G23-8 (anti-mouse IL-23 p19). IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted by activated monocytes, macrophages, and dendritic cells. IL-23 is a heterodimer composed of the disulfide-linked p19 protein and p40 protein, expressed by activated dendritic cells. Applications Reported: This C17.8 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This C17.8 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse thioglycolate-elicited peritoneal exudate cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to BestProtocols®: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity...
IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.
Specifications
IL-12/IL-23 p40 | |
Monoclonal | |
0.2 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
P43431, P43432, Q9EQ14 | |
IL12A, IL12B, Il23a | |
Affinity chromatography | |
RUO | |
16159, 16160, 83430 | |
4°C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
C17.8 | |
PE-Cyanine7 | |
IL12A | |
CLMF; CLMF p35; CLMF p40; CLMF2; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12/IL 23p40; il 23; IL 23 p40; Il12; Il-12 p35; IL12 p40; IL23; IL-12 subunit p35; IL-12 subunit p40; IL-12, subunit p35; IL12, subunit p40; Il12a; IL-12A; Il12b; IL-12B; IL12p35; IL-12p35; Il12p40; Il-12p40; il23; IL-23; IL23 p41; IL-23 subunit alpha; Il23a; IL-23A; IL-23-A; IL23P19; IL-23p19; IL35 subunit; ILN; IMD28; IMD29; Interleukin; Interleukin 12; interleukin 12 35 kDa subunit; interleukin 12 35kDa subunit; interleukin 12 40 kDa subunit; interleukin 12 40kDa subunit; interleukin 12 p35 subunit; interleukin 12 polypeptide 35; interleukin 12 subunit p40; interleukin 12, p35; interleukin 12, p40; interleukin 12A; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin 12b; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin 23 p19 subunit; interleukin 23 subunit alpha; interleukin 23, alpha subunit p19; Interleukin12; interleukin-12 alpha chain; interleukin-12 beta chain; interleukin-12 p40; interleukin-12 p40 subunit; interleukin-12 subunit alpha; Interleukin-12 subunit beta; interleukin-12 subunit p40; interleukin-23 p19 subunit; interleukin-23 p40 subunit; interleukin-23 subunit alpha; Interleukin-23 subunit p19; interleukin-six, G-CSF related factor; JKA3 induced upon T-cell activation; Ll12a; natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35; natural killer cell stimulatory factor, 40 kD subunit; NF cell stimulatory factor chain 1; NFSK; NK cell stimulatory factor chain 1; NK cell stimulatory factor chain 2; NKSF; NKSF1; NKSF2; p19; P35; p40; RP23-388G23.1; SGRF; UNQ2498/PRO5798 | |
Rat | |
100 μg | |
Primary | |
Mouse | |
Antibody | |
IgG2a κ |
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