Invitrogen™ Rat IL-13-Cell Lysates ELISA Kit

Catalog No.	Quantity
50-254-5354	10 x 96 Tests

Catalog No. 50-254-5354 Supplier Invitrogen™ Supplier No. ERIL13X10

Description

ELISA

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.

Specifications

• Accession Number: P42203
• Assay Range: 27.43 to 20,000 pg/mL
• Assay Sensitivity: 20 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Cell Lysates
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 116553
• Gene Symbol: Il13
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 20 μL
• Storage Requirements: 2°C to 8°C
• Target: IL-13
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins