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IL-2 Rat anti-Mouse, PE-Cyanine7, Clone: JES6-5H4, eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

$107.00 - $242.00

Specifications

Antigen IL-2
Clone JES6-5H4
Target Species Mouse
Conjugate PE-Cyanine7
Isotype IgG2b, kappa
View More Specs

Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-156-04 Non-distribution product as customer accommodation. View Documents Invitrogen
25702180
25 μg Each for $107.00
Add to cart
 
50-156-05 Non-distribution product as customer accommodation. View Documents Invitrogen
25702182
100 μg Each for $242.00
Add to cart
 
Description

Description

Description: The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine. Applications Reported: This JES6-5H4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This JES6-5H4 antibody has been tested by intracellular staining and flow cytrometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered. Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
Specifications

Specifications

IL-2
Mouse
IgG2b, kappa
Monoclonal
Liquid
Antibody
4° C, store in dark, DO NOT FREEZE!
JES6-5H4
PE-Cyanine7
Flow Cytometry
Primary
Monoclonal
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
RUO

For Research Use Only.

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