IL-21 Monoclonal Antibody (mhalx21), PE-Cyanine7, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
25-721-380	Invitrogen™ 25721380	25 μg
25-721-382	Invitrogen™ 25721382	100 μg

Description

Description: The monoclonal antibody mhalx21 reacts with mouse interleukin-21 (IL-21). IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by NKT, T helper (Th) 17 and T follicular helper (Tfh) cells. In Tfh cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1, IL-4 for Th2 and IL-17A for Th17, IL-21 is critical for Tfh cell effector function. This cytokine plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintece by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. High levels of IL-21 are present in chemically-induced colitis models. IL-21-deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules. Preliminary data suggest that clone mhalx21 recognizes a different epitope than anti-mouse IL-21 clone FFA21 (cat. 51-7211). Applications Reported: This mhalx21 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1 and IL-4 for Th2 cells, IL-21 is critical for TFH cell development and effector function. IL-21 plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. IL-21 deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules. In comparison, elevated levels of IL-21 are present in chemically-induced colitis mouse models.

Specifications

• Antigen: IL-21
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: CVID11; CX2; il 21; IL21; IL-21; ILN; Interleukin; interleukin 21; Interleukin21; interleukin-21; interleukin-21 isoform; porcine interleukin-21; Za11
• Gene Symbols: IL21
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: IL21

• Clone: mhalx21
• Applications: Flow Cytometry
• Conjugate: PE-Cyanine7
• Host Species: Rat
• Target Species: Mouse
• Gene Accession No.: Q9ES17
• Gene ID (Entrez): 60505
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody