Invitrogen™ IL-27 p28 Monoclonal Antibody (3D1p28), eFluor™ 660, eBioscience™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 50827742

Catalog No. 50-827-742

Description

Description: This 3D1p28 monoclonal antibody reacts with the human p28 subunit of IL-27. Interleukin-27 (IL-27) is a member of the IL-12 family, a subgroup of the IL-6 family of cytokines. It is a heterodimer of the subunits EBI3 (Epstein-Barr Virus Induced Gene 3), which is homologous to the p40 subunit shared by IL-12 and IL-23, and p28 (IL-30), which is homologous to p35. IL-27 is produced by activated dendritic cells and macrophages in response to TLR ligands and inflammatory cytokines. IL-27 has been shown to have both pro-inflammatory and anti-inflammatory effects. It influences the commitment of CD4+ T-cells toward the Th1 lineage by inducing the expression of the transcription factor T-bet and the upregulation of IL-12R beta2. Its anti-inflammatory functions include the suppression of Th2 and Th17 proliferation and differentiation. The IL-27 receptor shares one subunit, gp130, with other members of the IL-6 family. The subunit WSX-1 (IL-27R alpha, TCCR) is unique to IL-27 and is believed to be the only part of the receptor that interacts with the cytokine. IL-27R activation leads to the phosphorylation of Jak/STAT proteins, with STAT1 and STAT3 being critical to the function of IL-27. Recent evidence suggests that the p28 subunit may also be secreted independently of EBI3 and have functions distinct from the IL-27 heterodimer: it is believed to not only antagonize the activity of IL-27, but also inhibit signaling of other gp130 ligands, such as IL-6 and IL-11. Appl...

IL-27, a member of the IL-12 family, is a heterodimeric protein consisting of the p40-related protein Epstein-Barr virus-induced gene 3 (EBI3) non-covalently linked to an IL-12p35-related protein, p28 (also known as IL-30). IL-27 is produced by activated APCs and mature dendritic cells. IL-27 exerts its activities on NK cells and naive CD4+ T cells; mRNA expression analysis of IL-27 receptor components (WSX-1/TCCR and gp130) suggests that IL-27 may also target other cells, including mast cells and monocytes. Binding of IL-27 to WSX-1/gp130 activates JAK1, STAT1, and STAT3 and STAT1/3 phosphorylation. WSX-1/TCCR-deficient mice develop impaired Th1 responses and are more susceptible to infection with L. monocytogenes suggesting that Th1 responses require IL-27. Although activation of WSX-1 is required for the initiation of Th1 responses, it is not necessary for maintaining Th1 responses. IL-27 alone is not able to induce the differentiation of CD4+ T cells into IFN-γ-producing cells, suggesting a role for IL-27 as an initial activator of Th1 responses. An important effect of IL-27 in initiating Th1 responses is the induction of the Th1-specific transcription factor T-bet as well as the suppression of the Th2-specific transcription factor GATA-3. T-bet plays a critical role in Th1 differentiation by its ability to maintain IL-12Rβ2 expression following CD4+ T cell activation. Recent studies indicate that IL-27 has a potent antitumor activity. In vitro, IL-27 has been found to act directly on naive CD8 cells, generating CTL with enhanced granzyme B expression. In vivo, IL-27 has been reported to augment CTL activity, inhibit tumor growth, and induce complete regression of primary and metastatic neuroblastoma tumors.

Specifications

• Antigen: IL-27 p28
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q8NEV9
• Gene Symbols: Il27
• Immunogen: Recombinant IL-27.
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Flow Cytometry
• Clone: 3D1p28
• Conjugate: eFluor 660
• Gene: Il27
• Gene Alias: IL27; IL-27; IL-27 p28 subunit; IL-27 subunit alpha; Il27a; IL-27A; IL-27-A; IL27-A; IL27p28; IL-27p28; IL30; IL-30; ILN; Interleukin; interleukin 27; interleukin 30; Interleukin-27; interleukin-27 subunit alpha; Interleukin-30; p28
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 246778
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid