IL-4 Monoclonal Antibody (11B11), Functional Grade, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-146-96	Invitrogen 16704181	50 μg
50-146-97	Invitrogen 16704185	500 μg

Description

Description: The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kD cytokine. The 11B11 antibody is a neutralizing antibody. Mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surface MHC class II and CD23. Applications Reported: This 11B11 antibody has been reported for use in cytokine neutralization, ELISPOT, and ELISA cap. Applications Tested: The Functional Grade Purified 11B11 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-4 bioactivity. The 11B11 antibody at 0.125 μg/mL has been found to inhibit by 50% the biological effects of 1.0 ng/mL mouse IL-4 in a CTLL-2 cell proliferation assay. Please refer to Best Protocols for more information. The 11B11 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse interleukin-4 (IL-4) in combination with the biotinylated BVD6-24G2 (cat. 13-7042) antibody for detection and recombit mouse IL-4 (cat. 14-8041) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL.

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

Specifications

• Antigen: IL-4
• Concentration: 1 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with no preservative; pH 7.2
• Gene Alias: B cell growth factor 1; B_cell stimulatory factor 1; B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; cytokine; H-IL-4; IGG1 induction factor; IL2; IL4; Il-4; Il4e12; ILN; Interleukin; interleukin 4; interleukin 4 variant 2; interleukin 4 variant IL-4delta2; interleukin 4 variant IL-4delta3; interleukin 4 variant IL-4int2A; interleukin 4 variant IL-4int3A; interleukin 4 variant IL-4int3B; Interleukin4; interleukin-4; interleukin-4 precursor; lymphocyte stimulatory factor 1; MGC79402; M-IL-4; Pitrakinra
• Gene Symbols: Il4
• Primary or Secondary: Primary
• Content And Storage: 4° C
• Gene: Il4

• Clone: 11B11
• Applications: Functional Assay, Immunostaining
• Conjugate: Functional Grade
• Host Species: Rat
• Target Species: Mouse
• Gene Accession No.: P07750
• Gene ID (Entrez): 16189
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.