Invitrogen™ IL-5 Monoclonal Antibody (TRFK4), eBioscience™

Rat Monoclonal Antibody

Supplier:  Invitrogen™ 14705185

Catalog No. 50-136-11

Description

Description: The TRFK4 antibody reacts with mouse interleukin-5 (IL-5), also known as BCDF-alpha and eosinophil differentiation factor (EDF). This 15-20 kDa cytokine is secreted by Th2 cells and exists a homodimer. Applications Reported: TRFK4 has been reported for use in detection of mouse IL-5 by ELISA. Applications Tested: The Biotin conjugated TRFK4 has been tested by sandwich ELISA for detection of mouse IL-5 in combination with TRFK5 and can be used at 1 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.

Specifications

• Antigen: IL-5
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P04401
• Gene Symbols: Il5
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16191
• Content And Storage: 4°C
• Form: Liquid

• Applications: ELISA
• Clone: TRFK4
• Conjugate: Unconjugated
• Gene: Il5
• Gene Alias: B-cell differentiation factor I; B-cell growth factor II; BCGF-II; colony-stimulating factor, eosinophil; cytotoxic T-lymphocyte inducer; EDF; Eosinophil differentiation factor; IL5; IL-5; ILN; Interleukin; Interleukin 5; Interleukin 5 (colony-stimulating factor eosinophil); Interleukin 5 (colony-stimulating factor, eosinophil); Interleukin5; interleukin-5; T-cell replacing factor; TRF
• Host Species: Rat
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ