Invitrogen™ Human IL-6, Recombinant Protein

Description

Recombinant Protein

IL-6 (human) Recombinant Protein, RP-8619, from Invitrogen.

Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis. Recombinant human IL-6 is a non-glycosylated protein, containing 184 amino acids, with a molecular weight of 21 kDa. Purity is typically greater than 95% and endotoxin level less than 1 EU/ug. The expected biological activity (ED50) is determined by the dose-dependent stimulation of mouse 7TD1 cells and is typcially less than 1ng/mL. The specific activity of this protein is 1x10^6 units/mg. Reconstitute using sterile water at 0.1mg/mL and centrifuge prior to opening vial. Gently pipet solution down the sides of the vial. DO NOT VORTEX sample. Store reconstituted material at -80°C and add 0.1% BSA for additional stability. Amino acid sequence: PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM.

Specifications

• Accession Number: P05231
• Concentration: 0.1mg/mL
• For Use With (Application): Control
• Formulation: 0.01M sodium phosphate with no preservative
• Gene ID (Entrez): 3569
• Name: Human IL-6
• Quantity: 20 μg
• Source: E. Coli
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: H-IL-6
• Common Name: Human IL-6
• Gene Symbol: IL6
• Specific Reactivity: Human
• Conjugate: Unconjugated
• Cross Reactivity: Hu
• Species: E. coli
• Recombinant: Recombinant
• Form: Lyophilized
• Protein Subtype: Recombinant
• Purity or Quality Grade: purified